Construct: shRNA TRCN0000005083

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_034056.7-2740s1c1
DNA Barcode:
CCCGAGGAAATTAAGGACTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MED13L (23389)
Gene Description:
mediator complex subunit 13L
Transcript:
RefSeq XM_034056.7 (NON-CURRENT)
Match location:
Position 2740 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000005083)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCGAGGAAATTAAGGACTTT
Hairpin Sequence:
5'-CCGG-CCCGAGGAAATTAAGGACTTT-CTCGAG-AAAGTCCTTAATTTCCTCGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCGAGGAAATTAAGGACTTTCTCGAGAAAGTCCTTAATTTCCTCGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCGAGGAAATTAAGGACTTTCTCGAGAAAGTCCTTAATTTCCTCGGG-3'

Other clones with same target sequence:

(none)