Construct: shRNA TRCN0000005123

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015286.4-3121s1c1
DNA Barcode:
GCTGAAGTCAACGTCTCACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SYNM (23336)
Gene Description:
synemin
Transcript:
RefSeq NM_015286.4 (NON-CURRENT)
Match location:
Position 3121 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000005123)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGAAGTCAACGTCTCACAA
Hairpin Sequence:
5'-CCGG-GCTGAAGTCAACGTCTCACAA-CTCGAG-TTGTGAGACGTTGACTTCAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGAAGTCAACGTCTCACAACTCGAGTTGTGAGACGTTGACTTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGAAGTCAACGTCTCACAACTCGAGTTGTGAGACGTTGACTTCAGC-3'

Other clones with same target sequence:

  1. TRCN0000314852