Construct: shRNA TRCN0000005128

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022905.2-651s1c1
DNA Barcode:
GCGTAGCACTGACAAGAATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TTC23 (64927)
Gene Description:
tetratricopeptide repeat domain 23
Transcript:
RefSeq NM_022905.2 (NON-CURRENT)
Match location:
Position 651 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000005128)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGTAGCACTGACAAGAATTT
Hairpin Sequence:
5'-CCGG-GCGTAGCACTGACAAGAATTT-CTCGAG-AAATTCTTGTCAGTGCTACGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGTAGCACTGACAAGAATTTCTCGAGAAATTCTTGTCAGTGCTACGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGTAGCACTGACAAGAATTTCTCGAGAAATTCTTGTCAGTGCTACGC-3'

Other clones with same target sequence:

(none)