Construct: shRNA TRCN0000005132

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005587.1-2628s1c1
DNA Barcode:
GCCTAGAAATATAGAGCATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MEF2A (4205)
Gene Description:
myocyte enhancer factor 2A
Transcript:
RefSeq NM_005587.1 (NON-CURRENT)
Match location:
Position 2628 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000005132)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTAGAAATATAGAGCATTA
Hairpin Sequence:
5'-CCGG-GCCTAGAAATATAGAGCATTA-CTCGAG-TAATGCTCTATATTTCTAGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTAGAAATATAGAGCATTACTCGAGTAATGCTCTATATTTCTAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTAGAAATATAGAGCATTACTCGAGTAATGCTCTATATTTCTAGGC-3'

Other clones with same target sequence:

(none)