Construct: shRNA TRCN0000005152

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_291892.2-120s1c1
DNA Barcode:
CCTTCTCATCCTGATGGGTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OR2AT4 (341152)
Gene Description:
olfactory receptor family 2 subfamily AT member 4
Transcript:
RefSeq XM_291892.2 (NON-CURRENT)
Match location:
Position 120 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000005152)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTCTCATCCTGATGGGTAA
Hairpin Sequence:
5'-CCGG-CCTTCTCATCCTGATGGGTAA-CTCGAG-TTACCCATCAGGATGAGAAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTCTCATCCTGATGGGTAACTCGAGTTACCCATCAGGATGAGAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTCTCATCCTGATGGGTAACTCGAGTTACCCATCAGGATGAGAAGG-3'

Other clones with same target sequence:

(none)