Construct: shRNA TRCN0000005155

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007256.2-2909s1c1
DNA Barcode:
GCCTTTAAGAAGTGTCCCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLCO2B1 (11309)
Gene Description:
solute carrier organic anion transporter family member 2B1
Transcript:
RefSeq NM_007256.2 (NON-CURRENT)
Match location:
Position 2909 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000005155)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTTAAGAAGTGTCCCTTT
Hairpin Sequence:
5'-CCGG-GCCTTTAAGAAGTGTCCCTTT-CTCGAG-AAAGGGACACTTCTTAAAGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTTAAGAAGTGTCCCTTTCTCGAGAAAGGGACACTTCTTAAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTTAAGAAGTGTCCCTTTCTCGAGAAAGGGACACTTCTTAAAGGC-3'

Other clones with same target sequence:

(none)