Construct: shRNA TRCN0000005234

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004055.3-2400s1c1
DNA Barcode:
GCCAAGAAGATGTCACTTGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CAPN5 (726)
Gene Description:
calpain 5
Transcript:
RefSeq NM_004055.3 (NON-CURRENT)
Match location:
Position 2400 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000005234)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAAGAAGATGTCACTTGTT
Hairpin Sequence:
5'-CCGG-GCCAAGAAGATGTCACTTGTT-CTCGAG-AACAAGTGACATCTTCTTGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAAGAAGATGTCACTTGTTCTCGAGAACAAGTGACATCTTCTTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAAGAAGATGTCACTTGTTCTCGAGAACAAGTGACATCTTCTTGGC-3'

Other clones with same target sequence:

(none)