Construct: shRNA TRCN0000005311

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007086.1-2914s1c1
DNA Barcode:
CCCATTATAAAGCCTCTGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
WDHD1 (11169)
Gene Description:
WD repeat and HMG-box DNA binding protein 1
Transcript:
RefSeq NM_007086.1 (NON-CURRENT)
Match location:
Position 2914 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000005311)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCATTATAAAGCCTCTGATT
Hairpin Sequence:
5'-CCGG-CCCATTATAAAGCCTCTGATT-CTCGAG-AATCAGAGGCTTTATAATGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCATTATAAAGCCTCTGATTCTCGAGAATCAGAGGCTTTATAATGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCATTATAAAGCCTCTGATTCTCGAGAATCAGAGGCTTTATAATGGG-3'

Other clones with same target sequence:

(none)