Construct: shRNA TRCN0000005359

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152913.1-244s1c1
DNA Barcode:
GCCTGTATGAACTCAATCTCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TMEM130 (222865)
Gene Description:
transmembrane protein 130
Transcript:
RefSeq NM_152913.1 (NON-CURRENT)
Match location:
Position 244 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000005359)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTGTATGAACTCAATCTCA
Hairpin Sequence:
5'-CCGG-GCCTGTATGAACTCAATCTCA-CTCGAG-TGAGATTGAGTTCATACAGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTGTATGAACTCAATCTCACTCGAGTGAGATTGAGTTCATACAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTGTATGAACTCAATCTCACTCGAGTGAGATTGAGTTCATACAGGC-3'

Other clones with same target sequence:

(none)