Construct: shRNA TRCN0000005414

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032933.3-234s1c1
DNA Barcode:
AGACGCTCATTGGTGGACTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TMEM241 (85019)
Gene Description:
transmembrane protein 241
Transcript:
RefSeq NM_032933.3 (NON-CURRENT)
Match location:
Position 234 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000005414)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGACGCTCATTGGTGGACTTT
Hairpin Sequence:
5'-CCGG-AGACGCTCATTGGTGGACTTT-CTCGAG-AAAGTCCACCAATGAGCGTCT-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGACGCTCATTGGTGGACTTTCTCGAGAAAGTCCACCAATGAGCGTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGACGCTCATTGGTGGACTTTCTCGAGAAAGTCCACCAATGAGCGTCT-3'

Other clones with same target sequence:

(none)