Construct: shRNA TRCN0000005435

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173505.1-968s1c1
DNA Barcode:
TCCCTGAGAAACAAGGCCAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ANKRD29 (147463)
Gene Description:
ankyrin repeat domain 29
Transcript:
RefSeq NM_173505.1 (NON-CURRENT)
Match location:
Position 968 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000005435)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCCTGAGAAACAAGGCCAAT
Hairpin Sequence:
5'-CCGG-TCCCTGAGAAACAAGGCCAAT-CTCGAG-ATTGGCCTTGTTTCTCAGGGA-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCCTGAGAAACAAGGCCAATCTCGAGATTGGCCTTGTTTCTCAGGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCCTGAGAAACAAGGCCAATCTCGAGATTGGCCTTGTTTCTCAGGGA-3'

Other clones with same target sequence:

  1. TRCN0000273559