Construct: shRNA TRCN0000005452

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_290820.3-2044s1c1
DNA Barcode:
GAGTCTCAGAACGTCACCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SAMD4B (55095)
Gene Description:
sterile alpha motif domain containing 4B
Transcript:
RefSeq XM_290820.3 (NON-CURRENT)
Match location:
Position 2044 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000005452)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGTCTCAGAACGTCACCAAA
Hairpin Sequence:
5'-CCGG-GAGTCTCAGAACGTCACCAAA-CTCGAG-TTTGGTGACGTTCTGAGACTC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGTCTCAGAACGTCACCAAACTCGAGTTTGGTGACGTTCTGAGACTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGTCTCAGAACGTCACCAAACTCGAGTTTGGTGACGTTCTGAGACTC-3'

Other clones with same target sequence:

(none)