Construct: shRNA TRCN0000005505

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007345.1-2413s1c1
DNA Barcode:
CGTCCCTATATGTGTCCCTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF236 (7776)
Gene Description:
zinc finger protein 236
Transcript:
RefSeq NM_007345.1 (NON-CURRENT)
Match location:
Position 2413 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000005505)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTCCCTATATGTGTCCCTAT
Hairpin Sequence:
5'-CCGG-CGTCCCTATATGTGTCCCTAT-CTCGAG-ATAGGGACACATATAGGGACG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTCCCTATATGTGTCCCTATCTCGAGATAGGGACACATATAGGGACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTCCCTATATGTGTCCCTATCTCGAGATAGGGACACATATAGGGACG-3'

Other clones with same target sequence:

(none)