Construct: shRNA TRCN0000005527

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000790.1-243s1c1
DNA Barcode:
CGACGTTGAGAAGATAATCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DDC (1644)
Gene Description:
dopa decarboxylase
Transcript:
RefSeq NM_000790.1 (NON-CURRENT)
Match location:
Position 243 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000005527)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGACGTTGAGAAGATAATCAT
Hairpin Sequence:
5'-CCGG-CGACGTTGAGAAGATAATCAT-CTCGAG-ATGATTATCTTCTCAACGTCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGACGTTGAGAAGATAATCATCTCGAGATGATTATCTTCTCAACGTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGACGTTGAGAAGATAATCATCTCGAGATGATTATCTTCTCAACGTCG-3'

Other clones with same target sequence:

  1. TRCN0000335825