Construct: shRNA TRCN0000005533

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015198.2-978s1c1
DNA Barcode:
CCATGCACTCACGTTCTCTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
COBL (23242)
Gene Description:
cordon-bleu WH2 repeat protein
Transcript:
RefSeq NM_015198.2 (NON-CURRENT)
Match location:
Position 978 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000005533)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATGCACTCACGTTCTCTTA
Hairpin Sequence:
5'-CCGG-CCATGCACTCACGTTCTCTTA-CTCGAG-TAAGAGAACGTGAGTGCATGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATGCACTCACGTTCTCTTACTCGAGTAAGAGAACGTGAGTGCATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATGCACTCACGTTCTCTTACTCGAGTAAGAGAACGTGAGTGCATGG-3'

Other clones with same target sequence:

(none)