Construct: shRNA TRCN0000005536

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014302.2-209s1c1
DNA Barcode:
CAGCAATAGGATTTGCTATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SEC61G (23480)
Gene Description:
SEC61 translocon gamma subunit
Transcript:
RefSeq NM_014302.2 (NON-CURRENT)
Match location:
Position 209 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000005536)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGCAATAGGATTTGCTATAA
Hairpin Sequence:
5'-CCGG-CAGCAATAGGATTTGCTATAA-CTCGAG-TTATAGCAAATCCTATTGCTG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGCAATAGGATTTGCTATAACTCGAGTTATAGCAAATCCTATTGCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGCAATAGGATTTGCTATAACTCGAGTTATAGCAAATCCTATTGCTG-3'

Other clones with same target sequence:

  1. TRCN0000273571