Construct: shRNA TRCN0000005588

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005238.2-3566s1c1
DNA Barcode:
CCCAGAATTATCAGGAACATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ETS1 (2113)
Gene Description:
ETS proto-oncogene 1, transcription factor
Transcript:
RefSeq NM_005238.2 (NON-CURRENT)
Match location:
Position 3566 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000005588)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAGAATTATCAGGAACATA
Hairpin Sequence:
5'-CCGG-CCCAGAATTATCAGGAACATA-CTCGAG-TATGTTCCTGATAATTCTGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAGAATTATCAGGAACATACTCGAGTATGTTCCTGATAATTCTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAGAATTATCAGGAACATACTCGAGTATGTTCCTGATAATTCTGGG-3'

Other clones with same target sequence:

(none)