Construct: shRNA TRCN0000005684

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005997.1-1004s1c1
DNA Barcode:
GAGGCTTACAAGAAGTACATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
VPS72 (6944)
Gene Description:
vacuolar protein sorting 72 homolog
Transcript:
RefSeq NM_005997.1 (NON-CURRENT)
Match location:
Position 1004 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000005684)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGGCTTACAAGAAGTACATT
Hairpin Sequence:
5'-CCGG-GAGGCTTACAAGAAGTACATT-CTCGAG-AATGTACTTCTTGTAAGCCTC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGGCTTACAAGAAGTACATTCTCGAGAATGTACTTCTTGTAAGCCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGGCTTACAAGAAGTACATTCTCGAGAATGTACTTCTTGTAAGCCTC-3'

Other clones with same target sequence:

  1. TRCN0000335927