Construct: shRNA TRCN0000005713

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020770.1-3068s1c1
DNA Barcode:
CGGCTGGAAACAGAGTTAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CGN (57530)
Gene Description:
cingulin
Transcript:
RefSeq NM_020770.1 (NON-CURRENT)
Match location:
Position 3068 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000005713)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGCTGGAAACAGAGTTAGAT
Hairpin Sequence:
5'-CCGG-CGGCTGGAAACAGAGTTAGAT-CTCGAG-ATCTAACTCTGTTTCCAGCCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGCTGGAAACAGAGTTAGATCTCGAGATCTAACTCTGTTTCCAGCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGCTGGAAACAGAGTTAGATCTCGAGATCTAACTCTGTTTCCAGCCG-3'

Other clones with same target sequence:

(none)