Construct: shRNA TRCN0000005716

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020770.1-2001s1c1
DNA Barcode:
CCCGAGCTAGTGCTGGAGATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CGN (57530)
Gene Description:
cingulin
Transcript:
RefSeq NM_020770.1 (NON-CURRENT)
Match location:
Position 2001 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000005716)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCGAGCTAGTGCTGGAGATA
Hairpin Sequence:
5'-CCGG-CCCGAGCTAGTGCTGGAGATA-CTCGAG-TATCTCCAGCACTAGCTCGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCGAGCTAGTGCTGGAGATACTCGAGTATCTCCAGCACTAGCTCGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCGAGCTAGTGCTGGAGATACTCGAGTATCTCCAGCACTAGCTCGGG-3'

Other clones with same target sequence:

(none)