Construct: shRNA TRCN0000005731

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003855.2-1763s1c1
DNA Barcode:
GCTCACAATTTGAAGATGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IL18R1 (8809)
Gene Description:
interleukin 18 receptor 1
Transcript:
RefSeq NM_003855.2 (NON-CURRENT)
Match location:
Position 1763 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000005731)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCACAATTTGAAGATGAAA
Hairpin Sequence:
5'-CCGG-GCTCACAATTTGAAGATGAAA-CTCGAG-TTTCATCTTCAAATTGTGAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCACAATTTGAAGATGAAACTCGAGTTTCATCTTCAAATTGTGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCACAATTTGAAGATGAAACTCGAGTTTCATCTTCAAATTGTGAGC-3'

Other clones with same target sequence:

(none)