Construct: shRNA TRCN0000005761

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004257.3-2680s1c1
DNA Barcode:
CGAGTTCTTTCTGAGCCTGCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TGFBRAP1 (9392)
Gene Description:
transforming growth factor beta receptor associated protein 1
Transcript:
RefSeq NM_004257.3 (NON-CURRENT)
Match location:
Position 2680 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000005761)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGTTCTTTCTGAGCCTGCT
Hairpin Sequence:
5'-CCGG-CGAGTTCTTTCTGAGCCTGCT-CTCGAG-AGCAGGCTCAGAAAGAACTCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGTTCTTTCTGAGCCTGCTCTCGAGAGCAGGCTCAGAAAGAACTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGTTCTTTCTGAGCCTGCTCTCGAGAGCAGGCTCAGAAAGAACTCG-3'

Other clones with same target sequence:

(none)