Construct: shRNA TRCN0000005770

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024093.1-554s1c1
DNA Barcode:
CCATGACTTAACGCATAGGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C2orf49 (79074)
Gene Description:
chromosome 2 open reading frame 49
Transcript:
RefSeq NM_024093.1 (NON-CURRENT)
Match location:
Position 554 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000005770)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATGACTTAACGCATAGGAA
Hairpin Sequence:
5'-CCGG-CCATGACTTAACGCATAGGAA-CTCGAG-TTCCTATGCGTTAAGTCATGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATGACTTAACGCATAGGAACTCGAGTTCCTATGCGTTAAGTCATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATGACTTAACGCATAGGAACTCGAGTTCCTATGCGTTAAGTCATGG-3'

Other clones with same target sequence:

(none)