Construct: shRNA TRCN0000005775

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001450.3-710s1c1
DNA Barcode:
CCAGGAATGCAAGAAGACCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FHL2 (2274)
Gene Description:
four and a half LIM domains 2
Transcript:
RefSeq NM_001450.3 (NON-CURRENT)
Match location:
Position 710 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000005775)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGGAATGCAAGAAGACCAT
Hairpin Sequence:
5'-CCGG-CCAGGAATGCAAGAAGACCAT-CTCGAG-ATGGTCTTCTTGCATTCCTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGGAATGCAAGAAGACCATCTCGAGATGGTCTTCTTGCATTCCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGGAATGCAAGAAGACCATCTCGAGATGGTCTTCTTGCATTCCTGG-3'

Other clones with same target sequence:

(none)