Construct: shRNA TRCN0000005886

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002451.2-313s1c1
DNA Barcode:
GCACACCATCATGCCTTCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MTAP (4507)
Gene Description:
methylthioadenosine phosphorylase
Transcript:
RefSeq NM_002451.2 (NON-CURRENT)
Match location:
Position 313 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000005886)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACACCATCATGCCTTCAAA
Hairpin Sequence:
5'-CCGG-GCACACCATCATGCCTTCAAA-CTCGAG-TTTGAAGGCATGATGGTGTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACACCATCATGCCTTCAAACTCGAGTTTGAAGGCATGATGGTGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACACCATCATGCCTTCAAACTCGAGTTTGAAGGCATGATGGTGTGC-3'

Other clones with same target sequence:

(none)