Construct: shRNA TRCN0000005892

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021616.3-519s1c1
DNA Barcode:
CGGAGGAAGTATTCAAGGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TRIM34 (53840)
Gene Description:
tripartite motif containing 34
Transcript:
RefSeq NM_021616.3 (NON-CURRENT)
Match location:
Position 519 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000005892)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGAGGAAGTATTCAAGGAAT
Hairpin Sequence:
5'-CCGG-CGGAGGAAGTATTCAAGGAAT-CTCGAG-ATTCCTTGAATACTTCCTCCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGAGGAAGTATTCAAGGAATCTCGAGATTCCTTGAATACTTCCTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGAGGAAGTATTCAAGGAATCTCGAGATTCCTTGAATACTTCCTCCG-3'

Other clones with same target sequence:

(none)