Construct: shRNA TRCN0000005934

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003844.2-1499s1c1
DNA Barcode:
CTTAGGTGTTAGGAGTTAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TNFRSF10A (8797)
Gene Description:
TNF receptor superfamily member 10a
Transcript:
RefSeq NM_003844.2 (NON-CURRENT)
Match location:
Position 1499 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000005934)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTAGGTGTTAGGAGTTAATA
Hairpin Sequence:
5'-CCGG-CTTAGGTGTTAGGAGTTAATA-CTCGAG-TATTAACTCCTAACACCTAAG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTAGGTGTTAGGAGTTAATACTCGAGTATTAACTCCTAACACCTAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTAGGTGTTAGGAGTTAATACTCGAGTATTAACTCCTAACACCTAAG-3'

Other clones with same target sequence:

(none)