Construct: shRNA TRCN0000005963

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017714.1-1625s1c1
DNA Barcode:
CCTCATTGTGTGACCAGGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TASP1 (55617)
Gene Description:
taspase 1
Transcript:
RefSeq NM_017714.1 (NON-CURRENT)
Match location:
Position 1625 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000005963)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCATTGTGTGACCAGGAAT
Hairpin Sequence:
5'-CCGG-CCTCATTGTGTGACCAGGAAT-CTCGAG-ATTCCTGGTCACACAATGAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCATTGTGTGACCAGGAATCTCGAGATTCCTGGTCACACAATGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCATTGTGTGACCAGGAATCTCGAGATTCCTGGTCACACAATGAGG-3'

Other clones with same target sequence:

(none)