Construct: shRNA TRCN0000005972
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_005053.2-1232s1c1
- DNA Barcode:
- CCCTACCCTTATTCCATGAAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- RAD23A (5886)
- Gene Description:
- RAD23 homolog A, nucleotide excision repair protein
- Transcript:
- RefSeq NM_005053.2 (NON-CURRENT)
- Match location:
- Position 1232 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000005972)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCCTACCCTTATTCCATGAAA
- Hairpin Sequence:
-
5'-CCGG-CCCTACCCTTATTCCATGAAA-CTCGAG-TTTCATGGAATAAGGGTAGGG-TTTTT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCCTACCCTTATTCCATGAAACTCGAGTTTCATGGAATAAGGGTAGGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCCTACCCTTATTCCATGAAACTCGAGTTTCATGGAATAAGGGTAGGG-3'
Other clones with same target sequence:
-
TRCN0000318421
