Construct: shRNA TRCN0000005979

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003671.2-1183s1c1
DNA Barcode:
CTCCTGAGACTTATATTCAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDC14B (8555)
Gene Description:
cell division cycle 14B
Transcript:
RefSeq NM_003671.2 (NON-CURRENT)
Match location:
Position 1183 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000005979)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCCTGAGACTTATATTCAAT
Hairpin Sequence:
5'-CCGG-CTCCTGAGACTTATATTCAAT-CTCGAG-ATTGAATATAAGTCTCAGGAG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCCTGAGACTTATATTCAATCTCGAGATTGAATATAAGTCTCAGGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCCTGAGACTTATATTCAATCTCGAGATTGAATATAAGTCTCAGGAG-3'

Other clones with same target sequence:

  1. TRCN0000273454