Construct: shRNA TRCN0000006064

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005316.2-2502s1c1
DNA Barcode:
CCTGAATAATAAAGAGGGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GTF2H1 (2965)
Gene Description:
general transcription factor IIH subunit 1
Transcript:
RefSeq NM_005316.2 (NON-CURRENT)
Match location:
Position 2502 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000006064)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGAATAATAAAGAGGGAAT
Hairpin Sequence:
5'-CCGG-CCTGAATAATAAAGAGGGAAT-CTCGAG-ATTCCCTCTTTATTATTCAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGAATAATAAAGAGGGAATCTCGAGATTCCCTCTTTATTATTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGAATAATAAAGAGGGAATCTCGAGATTCCCTCTTTATTATTCAGG-3'

Other clones with same target sequence:

(none)