Construct: shRNA TRCN0000006069

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004196.3-706s1c1
DNA Barcode:
CCAGCAAGTGTTTAGCACGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDKL1 (8814)
Gene Description:
cyclin dependent kinase like 1
Transcript:
RefSeq NM_004196.3 (NON-CURRENT)
Match location:
Position 706 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000006069)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGCAAGTGTTTAGCACGAA
Hairpin Sequence:
5'-CCGG-CCAGCAAGTGTTTAGCACGAA-CTCGAG-TTCGTGCTAAACACTTGCTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGCAAGTGTTTAGCACGAACTCGAGTTCGTGCTAAACACTTGCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGCAAGTGTTTAGCACGAACTCGAGTTCGTGCTAAACACTTGCTGG-3'

Other clones with same target sequence:

(none)