Construct: shRNA TRCN0000006100

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017572.2-1172s1c1
DNA Barcode:
GCCTGCCAGAACATGCTGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MKNK2 (2872)
Gene Description:
MAPK interacting serine/threonine kinase 2
Transcript:
RefSeq NM_017572.2 (NON-CURRENT)
Match location:
Position 1172 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000006100)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTGCCAGAACATGCTGTTT
Hairpin Sequence:
5'-CCGG-GCCTGCCAGAACATGCTGTTT-CTCGAG-AAACAGCATGTTCTGGCAGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTGCCAGAACATGCTGTTTCTCGAGAAACAGCATGTTCTGGCAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTGCCAGAACATGCTGTTTCTCGAGAAACAGCATGTTCTGGCAGGC-3'

Other clones with same target sequence:

(none)