Construct: shRNA TRCN0000006158

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_042066.8-5517s1c1
DNA Barcode:
CCACTCTTATTGTGCAGGTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAP3K1 (4214)
Gene Description:
mitogen-activated protein kinase kinase kinase 1
Transcript:
RefSeq XM_042066.8 (NON-CURRENT)
Match location:
Position 5517 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000006158)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACTCTTATTGTGCAGGTTA
Hairpin Sequence:
5'-CCGG-CCACTCTTATTGTGCAGGTTA-CTCGAG-TAACCTGCACAATAAGAGTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACTCTTATTGTGCAGGTTACTCGAGTAACCTGCACAATAAGAGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACTCTTATTGTGCAGGTTACTCGAGTAACCTGCACAATAAGAGTGG-3'

Other clones with same target sequence:

(none)