Construct: shRNA TRCN0000006205

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006213.2-1178s1c1
DNA Barcode:
GCCTACGCTTTCCGAATCTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PHKG1 (5260)
Gene Description:
phosphorylase kinase catalytic subunit gamma 1
Transcript:
RefSeq NM_006213.2 (NON-CURRENT)
Match location:
Position 1178 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000006205)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTACGCTTTCCGAATCTAT
Hairpin Sequence:
5'-CCGG-GCCTACGCTTTCCGAATCTAT-CTCGAG-ATAGATTCGGAAAGCGTAGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTACGCTTTCCGAATCTATCTCGAGATAGATTCGGAAAGCGTAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTACGCTTTCCGAATCTATCTCGAGATAGATTCGGAAAGCGTAGGC-3'

Other clones with same target sequence:

(none)