Construct: shRNA TRCN0000006207

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001787.1-2043s1c1
DNA Barcode:
CGATCAGATCAACAAGGTGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDK11B (984)
Gene Description:
cyclin dependent kinase 11B
Transcript:
RefSeq NM_001787.1 (NON-CURRENT)
Match location:
Position 2043 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000006207)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGATCAGATCAACAAGGTGTT
Hairpin Sequence:
5'-CCGG-CGATCAGATCAACAAGGTGTT-CTCGAG-AACACCTTGTTGATCTGATCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGATCAGATCAACAAGGTGTTCTCGAGAACACCTTGTTGATCTGATCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGATCAGATCAACAAGGTGTTCTCGAGAACACCTTGTTGATCTGATCG-3'

Other clones with same target sequence:

  1. TRCN0000314694