Construct: shRNA TRCN0000006211

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005713.1-1059s1c1
DNA Barcode:
GCATAGTACCAACGGCAATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CERT1 (10087)
Gene Description:
ceramide transporter 1
Transcript:
RefSeq NM_005713.1 (NON-CURRENT)
Match location:
Position 1059 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000006211)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATAGTACCAACGGCAATAA
Hairpin Sequence:
5'-CCGG-GCATAGTACCAACGGCAATAA-CTCGAG-TTATTGCCGTTGGTACTATGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATAGTACCAACGGCAATAACTCGAGTTATTGCCGTTGGTACTATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATAGTACCAACGGCAATAACTCGAGTTATTGCCGTTGGTACTATGC-3'

Other clones with same target sequence:

(none)