Construct: shRNA TRCN0000006221

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013302.2-3074s1c1
DNA Barcode:
CCCATAAATGACAGTGACTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EEF2K (29904)
Gene Description:
eukaryotic elongation factor 2 kinase
Transcript:
RefSeq NM_013302.2 (NON-CURRENT)
Match location:
Position 3074 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000006221)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCATAAATGACAGTGACTTT
Hairpin Sequence:
5'-CCGG-CCCATAAATGACAGTGACTTT-CTCGAG-AAAGTCACTGTCATTTATGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCATAAATGACAGTGACTTTCTCGAGAAAGTCACTGTCATTTATGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCATAAATGACAGTGACTTTCTCGAGAAAGTCACTGTCATTTATGGG-3'

Other clones with same target sequence:

(none)