Construct: shRNA TRCN0000006227

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_290793.4-2340s1c1
DNA Barcode:
GCTCTTCAAGAAAGAGGTGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KSR1 (8844)
Gene Description:
kinase suppressor of ras 1
Transcript:
RefSeq XM_290793.4 (NON-CURRENT)
Match location:
Position 2340 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000006227)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCTTCAAGAAAGAGGTGAT
Hairpin Sequence:
5'-CCGG-GCTCTTCAAGAAAGAGGTGAT-CTCGAG-ATCACCTCTTTCTTGAAGAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCTTCAAGAAAGAGGTGATCTCGAGATCACCTCTTTCTTGAAGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCTTCAAGAAAGAGGTGATCTCGAGATCACCTCTTTCTTGAAGAGC-3'

Other clones with same target sequence:

  1. TRCN0000338415