Construct: shRNA TRCN0000006241

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002595.2-2366s1c1
DNA Barcode:
GCGGAATCATTGCTGCTAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDK17 (5128)
Gene Description:
cyclin dependent kinase 17
Transcript:
RefSeq NM_002595.2 (NON-CURRENT)
Match location:
Position 2366 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000006241)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGGAATCATTGCTGCTAAAT
Hairpin Sequence:
5'-CCGG-GCGGAATCATTGCTGCTAAAT-CTCGAG-ATTTAGCAGCAATGATTCCGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGGAATCATTGCTGCTAAATCTCGAGATTTAGCAGCAATGATTCCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGGAATCATTGCTGCTAAATCTCGAGATTTAGCAGCAATGATTCCGC-3'

Other clones with same target sequence:

  1. TRCN0000297344