Construct: shRNA TRCN0000006250

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006256.1-3041s1c1
DNA Barcode:
GCCACCAATAGCTTCTGAGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PKN2 (5586)
Gene Description:
protein kinase N2
Transcript:
RefSeq NM_006256.1 (NON-CURRENT)
Match location:
Position 3041 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000006250)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCACCAATAGCTTCTGAGTT
Hairpin Sequence:
5'-CCGG-GCCACCAATAGCTTCTGAGTT-CTCGAG-AACTCAGAAGCTATTGGTGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCACCAATAGCTTCTGAGTTCTCGAGAACTCAGAAGCTATTGGTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCACCAATAGCTTCTGAGTTCTCGAGAACTCAGAAGCTATTGGTGGC-3'

Other clones with same target sequence:

(none)