Construct: shRNA TRCN0000006261

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002610.3-1271s1c1
DNA Barcode:
GCTCTGTCAACAGACTCAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PDK1 (5163)
Gene Description:
pyruvate dehydrogenase kinase 1
Transcript:
RefSeq NM_002610.3 (NON-CURRENT)
Match location:
Position 1271 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000006261)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCTGTCAACAGACTCAATA
Hairpin Sequence:
5'-CCGG-GCTCTGTCAACAGACTCAATA-CTCGAG-TATTGAGTCTGTTGACAGAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCTGTCAACAGACTCAATACTCGAGTATTGAGTCTGTTGACAGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCTGTCAACAGACTCAATACTCGAGTATTGAGTCTGTTGACAGAGC-3'

Other clones with same target sequence:

  1. TRCN0000342426