Construct: shRNA TRCN0000006287

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004606.2-4753s1c1
DNA Barcode:
CCAATGGATTTAGAGACCATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TAF1 (6872)
Gene Description:
TATA-box binding protein associated factor 1
Transcript:
RefSeq NM_004606.2 (NON-CURRENT)
Match location:
Position 4753 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000006287)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAATGGATTTAGAGACCATA
Hairpin Sequence:
5'-CCGG-CCAATGGATTTAGAGACCATA-CTCGAG-TATGGTCTCTAAATCCATTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAATGGATTTAGAGACCATACTCGAGTATGGTCTCTAAATCCATTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAATGGATTTAGAGACCATACTCGAGTATGGTCTCTAAATCCATTGG-3'

Other clones with same target sequence:

(none)