Construct: shRNA TRCN0000006294

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006255.2-2781s1c1
DNA Barcode:
GCTTCTTAATTCAAGAGACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRKCH (5583)
Gene Description:
protein kinase C eta
Transcript:
RefSeq NM_006255.2 (NON-CURRENT)
Match location:
Position 2781 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000006294)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTCTTAATTCAAGAGACAA
Hairpin Sequence:
5'-CCGG-GCTTCTTAATTCAAGAGACAA-CTCGAG-TTGTCTCTTGAATTAAGAAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTCTTAATTCAAGAGACAACTCGAGTTGTCTCTTGAATTAAGAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTCTTAATTCAAGAGACAACTCGAGTTGTCTCTTGAATTAAGAAGC-3'

Other clones with same target sequence:

(none)