Construct: shRNA TRCN0000006302

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012096.1-2130s1c1
DNA Barcode:
GCCAGTCAGAAGAGAGTGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
APPL1 (26060)
Gene Description:
adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1
Transcript:
RefSeq NM_012096.1 (NON-CURRENT)
Match location:
Position 2130 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000006302)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAGTCAGAAGAGAGTGATT
Hairpin Sequence:
5'-CCGG-GCCAGTCAGAAGAGAGTGATT-CTCGAG-AATCACTCTCTTCTGACTGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAGTCAGAAGAGAGTGATTCTCGAGAATCACTCTCTTCTGACTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAGTCAGAAGAGAGTGATTCTCGAGAATCACTCTCTTCTGACTGGC-3'

Other clones with same target sequence:

  1. TRCN0000315404