Construct: shRNA TRCN0000006317

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007174.1-3603s1c1
DNA Barcode:
GCTGATCTACTGAAGACAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CIT (11113)
Gene Description:
citron rho-interacting serine/threonine kinase
Transcript:
RefSeq NM_007174.1 (NON-CURRENT)
Match location:
Position 3603 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000006317)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGATCTACTGAAGACAGAA
Hairpin Sequence:
5'-CCGG-GCTGATCTACTGAAGACAGAA-CTCGAG-TTCTGTCTTCAGTAGATCAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGATCTACTGAAGACAGAACTCGAGTTCTGTCTTCAGTAGATCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGATCTACTGAAGACAGAACTCGAGTTCTGTCTTCAGTAGATCAGC-3'

Other clones with same target sequence:

  1. TRCN0000277748