Construct: shRNA TRCN0000006359

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001721.3-2169s1c1
DNA Barcode:
GAGTGCTGATAAGAATGAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BMX (660)
Gene Description:
BMX non-receptor tyrosine kinase
Transcript:
RefSeq NM_001721.3 (NON-CURRENT)
Match location:
Position 2169 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000006359)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGTGCTGATAAGAATGAATA
Hairpin Sequence:
5'-CCGG-GAGTGCTGATAAGAATGAATA-CTCGAG-TATTCATTCTTATCAGCACTC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGTGCTGATAAGAATGAATACTCGAGTATTCATTCTTATCAGCACTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGTGCTGATAAGAATGAATACTCGAGTATTCATTCTTATCAGCACTC-3'

Other clones with same target sequence:

(none)