Construct: shRNA TRCN0000006472

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006098.2-297s1c1
DNA Barcode:
GATGTGGTTATCTCCTCAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RACK1 (10399)
Gene Description:
receptor for activated C kinase 1
Transcript:
RefSeq NM_006098.2 (NON-CURRENT)
Match location:
Position 297 (CDS)
CRISPR Mechanism
CRISPR

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000006472)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGTGGTTATCTCCTCAGAT
Hairpin Sequence:
5'-CCGG-GATGTGGTTATCTCCTCAGAT-CTCGAG-ATCTGAGGAGATAACCACATC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGTGGTTATCTCCTCAGATCTCGAGATCTGAGGAGATAACCACATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGTGGTTATCTCCTCAGATCTCGAGATCTGAGGAGATAACCACATC-3'

Other clones with same target sequence:

  1. TRCN0000273093