Construct: shRNA TRCN0000006499

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006156.1-239s1c1
DNA Barcode:
GCAAGCAGATGAATGATGAGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NEDD8 (4738)
Gene Description:
NEDD8 ubiquitin like modifier
Transcript:
RefSeq NM_006156.1 (NON-CURRENT)
Match location:
Position 239 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000006499)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAGCAGATGAATGATGAGA
Hairpin Sequence:
5'-CCGG-GCAAGCAGATGAATGATGAGA-CTCGAG-TCTCATCATTCATCTGCTTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAGCAGATGAATGATGAGACTCGAGTCTCATCATTCATCTGCTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAGCAGATGAATGATGAGACTCGAGTCTCATCATTCATCTGCTTGC-3'

Other clones with same target sequence:

(none)