Construct: shRNA TRCN0000006507

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005427.1-2076s1c1
DNA Barcode:
CTCTCCTTCCTGTGTGTCCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TP73 (7161)
Gene Description:
tumor protein p73
Transcript:
RefSeq NM_005427.1 (NON-CURRENT)
Match location:
Position 2076 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000006507)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCTCCTTCCTGTGTGTCCAA
Hairpin Sequence:
5'-CCGG-CTCTCCTTCCTGTGTGTCCAA-CTCGAG-TTGGACACACAGGAAGGAGAG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCTCCTTCCTGTGTGTCCAACTCGAGTTGGACACACAGGAAGGAGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCTCCTTCCTGTGTGTCCAACTCGAGTTGGACACACAGGAAGGAGAG-3'

Other clones with same target sequence:

  1. TRCN0000272527